Molecular detection and phylogenetic analysis of Citrobacter freundii isolates from broilers in AL- Diwaniyah province of Iraq

Because of the medicinal and economic importance Citrobacter freundii, as well fact that there are only a few local studies on it, purpose this study was to isolate identify important bacterial species from chicken using differential media with comparable biochemical properties. There is severe concern about presence extended-spectrum beta-lactamase (ESBL) creating bacteria in animal husbandry, which could be transmitted humans. C. freundii detected through use specific associated genes enable bacterium resist effects antibiotics, making it highly resistant. For detection recognition distinct types resistance genes, polymerase chain reaction used. Further confirmation, purified PCR product subjected sequencing. It showed 99.80% homology Switzerland isolates available NCBI database. Forty two were isolated (450) fecal sample collected different region AL- Diwaniyah province.